Replication of Grapevine Fanleaf Virus Satellite RNA Transcripts in Chenopodium quinoa Protoplasts
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چکیده
منابع مشابه
Replication of grapevine fanleaf virus satellite RNA transcripts in Chenopodium quinoa protoplasts.
A set of full-length cDNA clones of the satellite RNA of grapevine fanleaf nepovirus isolate F13 (GFLV-F13) was constructed with a variable number of additional, non-viral nucleotides at the 5' and 3' ends. The biological activity of the RNAs transcribed from these constructs was tested in Chenopodium quinoa protoplasts using a helper virus. When inoculated with arabis mosaic virus S (ArMV-S) R...
متن کاملImmunodetection of grapevine fanleaf virus satellite RNA-encoded protein in infected Chenopodium quinoa.
An antiserum was raised against a fusion protein containing the C-terminal half of the protein (P3) encoded by the satellite RNA of grapevine fanleaf virus (GFLV; F13 isolate) and the N-terminal portion of the CI repressor of phage lambda. This antiserum specifically recognized P3 synthesized in the in vitro wheatgerm translation system and also in infected Chenopodium quinoa plants. In these p...
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Infection by Grapevine fanleaf nepovirus (GFLV), a bipartite RNA virus of positive polarity belonging to the Comoviridae family, causes extensive cytopathic modifications of the host endomembrane system that eventually culminate in the formation of a perinuclear "viral compartment." We identified by immunoconfocal microscopy this compartment as the site of virus replication since it contained t...
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The complete sequences of RNA1, RNA2 and satellite RNA have been determined for a South African isolate of Grapevine fanleaf virus (GFLV-SACH44). The two RNAs of GFLV-SACH44 are 7,341 nucleotides (nt) and 3,816 nt in length, respectively, and its satellite RNA (satRNA) is 1,104 nt in length, all excluding the poly(A) tail. Multiple sequence alignment of these sequences showed that GFLV-SACH44 R...
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Grapevine degeneration in grapevines caused by Grapevine fanleaf virus (GFLV) has been documented in many viticultural regions worldwide (Andret-Link et al. 2004). It is one of the major economically important virus diseases affecting the longevity of grapevines and reducing the fruit yield and fruit quality. Infected grapevines show a range of foliar symptoms consisting of leaf deformation, ye...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1992
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-73-10-2517